Native Instincts Kicked In

Well I can say I have a green thumb. The pea plant seeds came out with all pots successfully growing into sprouts. Figure 1 below shows the 100% success of all pots growing pea plants. Furthermore, the green tray was split with my lab made divider using yellow tape and cotton balls. I split the tray so the plants wouldn’t share the liquid of DI water and faucet water, but it turned out that both liquids different in pH both grew the plants. I think my last plants didn’t grow because the temperature was at 34 degrees Celsius; also, the soil was soaked rather than lightly moisten and the mixed soil with vermiculite.

Figure 1. The bottom left corner shows a close up of the success. There is a yellow arrow showing the yellow tape dividing the two rows of pots.

I, also, sampled two locations off campus for agrobacterium tumefaciens (A.tumefaciens). Figure 2 below show the samples in 0.1% Peptone of 45mL with the corresponding test tubes. Once the sample was chiseled out with sprayed 70% alcohol of a hammer, flat head screwdriver, and chisel, it was immediately put into a urine sample cup of Peptone of 90mL for isolation of bacteria. The piece of the crown gall was then rinsed with sterile DI water then cut using a sterile scalpel and tweezers. This whole procedure was performed in the flow hood for low risk of contamination. The pieces cut were place in two sterile test tubes of 6mL of Peptone and two sterile test tubes of 6mL of Tryptic Soy Broth (TSB). Then, the eight test tubes were stored in a cabinet for room temperature of 25 degree Celsius for proper growth of A.tumefaciens.

Figure 2. The neon green top rows of test tubes are tree "A." The next 2 bottom yellow rows are tree "B." Basically, the top row has TSB. The next row has Peptone, then the next row is TSB. The final row has Peptone.