Thursday, November 7, 2013

Interesting Article


I found a simple formula for how to calculate the sampling sites of a location.

Although the whole article was based on the pharmaceutical industry of manufacturing healthcare products, the whole concept of using contact plates is to make the sampling easier and quicker for my project. The previous semester of using swabs and sterile tubes was a long process from swabbing to inoculating to TSA media for incubation of CFU. It would take about almost two hours just to sample an area with the swabs, but now the contact plates make it easier to take samples. In other words, the PDF article was beneficial on how to sample areas of potential contamination of microorganisms. It, also, explains the proper procedures to take when analyzing the results of the incubated plates after 24 hours. The simple equation above used in a real situation would be used like the example below.
 The room used would be the DB-Building lab room 107 of the bottom floor.
Figure 1. Shows the location of sampling to be taken.

Figure 2. Shows the idea of how to calculate the minimum number of sampling locations.

Where, NL is the minimum number of sampling locations (rounded up to a whole number)
A is the area of the clean room or zone in meters2.
Article Link

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