Friday, February 7, 2014

All This Reading for Nothing

Diagram1. This poor diagram shows the mechanism of how helium used an force to push the particles of gold coated with the desired DNA in the syringe filter shoots into target of plant tissue. The purple oval shaped circles above the box is where the solenoid would go and the other two is the pressure gauges within the  box.
I started reading an article I found about developing a simple gene gun apparatuses. It included welding pieces of 0.5 centimeters (cm) thick stainless steel sheets into a cube like shape. The open side was covered with 1.5cm thick Plexiglas with 1.0cm thick rubber gasket around the edges. The thick Plexiglas would serve as a door to the cube. Thus, the overall specifications would have a solenoid attached to the top which it served as the mechanism of the gun. Diagram 1 above shows the specifications of the simple gene gun. The vacuum served to silent the nose of the compressed helium shooting thru the shaft of the micro tube of the solenoid. The coated gold particles with the desired genes are shot at a very high acceleration to intervene with the nucleus of the plant cell without disrupting its normal function. In conclusion, the desired DNA is inserted into the DNA of the plant DNA if it was cut in some random area. This will overall make the DNA code for some kind of gene expression.  

Josh on the other hand had a cheaper and less efficient way of making a gene gun with a super soaker. Same concept but cheaper parts and instead of gold for its particle to be coated it will be the chemical powder tungsten. Also, the gas helium would be switched out for nitrogen and carbon dioxide powder.

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