I started reading an article I found about
developing a simple gene gun apparatuses. It included welding pieces of 0.5
centimeters (cm) thick stainless steel sheets into a cube like shape. The open
side was covered with 1.5cm thick Plexiglas with 1.0cm thick rubber gasket
around the edges. The thick Plexiglas would serve as a door to the cube. Thus,
the overall specifications would have a solenoid attached to the top which it
served as the mechanism of the gun. Diagram 1 above shows the specifications of
the simple gene gun. The vacuum served to silent the nose of the compressed
helium shooting thru the shaft of the micro tube of the solenoid. The coated
gold particles with the desired genes are shot at a very high acceleration to
intervene with the nucleus of the plant cell without disrupting its normal
function. In conclusion, the desired DNA is inserted into the DNA of the plant
DNA if it was cut in some random area. This will overall make the DNA code for
some kind of gene expression.
Josh on the other hand had a cheaper and less efficient
way of making a gene gun with a super soaker. Same concept but cheaper parts
and instead of gold for its particle to be coated it will be the chemical
powder tungsten. Also, the gas helium would be switched out for nitrogen and
carbon dioxide powder.
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