Thursday, September 19, 2013

Organized and Ready


I started this week off with a great start. I have a good amount of contact plates with no problems of loss of inventory. I prepared about six sleeves of 20 plates this week. Figure 1 below shows the progress of perfect dome shaped above the rim of the plates being prepared. I modified the dispensing of 17mL to 16mL because of the overflow and loss of plates.
Figure 1. The plates are being UV for further sterilization.

Figure 2. The numbers correspond to the table 1 below of total Colony Forming Units.
Plate # Total CFU Plate # Total CFU Plate # Total CFU Plate # Total CFU
1 89 6 82 11 54 16 19
2 6 7 98 12 47 17 31
3 19 8 65 13 31 18 29
4 49 9 62 14 26 19 12
5 30 10 39 15 18 20 35

I, also, sampled the plates on two locations around campus. The first location was room 221 of the DB building on the second floor. Figure 2 above shows the area being monitored for fomites and the corresponding plates to CFU’s. I noticed that as you move from the back to the front of the room the CFU's get smaller per plate.

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