A few days ago, I took out my door handle cultures,
and I compared them and quantified the colonies growing. Figure 1 and 2 is the external
door handles, and figure 3 and 4 is the internal handles of the 2nd
floor classrooms of the DB building. I’m waiting and planning to gram stain
them soon. One of the lab sith lords are pushing me to stain. I won’t say whom. I'm looking forward to it but never have time.
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Figure 1. Shows the TSA plates being incubated for 3 days; it was supposed to be for 24 hours. |
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Figure 2. Shows only six because I swab a public door, so it was excluded from my data for being a public door. |
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Figure 3. The before shot of potential microbes. |
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Figure 4. The plate number 14 is excluded because of same reason above, and I just wanted to show the ones that did have something. Number 4 has like a splash of growing cultures instead of circular. |
Next
week in microbiology is gram staining, microscopy, and smear preparation; my
purpose for gram staining is identifying the cell wall of present bacteria
growing in my plates. The internship is really giving me some pre-knowledge of
the future labs. For instance, the pre-labs are, also, going over the
microscopic imaging of the shapes and sizes, and the anaerobic and
non-anaerobic that prokaryotic cells need. This will lead to a key of some
sort, a bible for identifying bacteria, fungi, and other microbes.
I
started a small project and took samples of my mouth, hat, and forehead. How
much microorganisms are present in my mouth, hat, and forehead? The results
were awesome. The TSA plates lit up with orange dots everywhere. Figure 5-6
show the results of a before and after shot. The mouth swab culture looks like
smeared mustard, and the forehead swabbing looks like sprinkled yellow salt
grains. Lastly, the hast swab looks like the above sample of the classroom door handles.
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Figure 5. Before entering the incubator for 3 days. |
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Figure 6. Looks like yellow mustard. Can't wait to gram stain this one. |
Today,
the second target being swabbed is rails. Figure 7-8 shows the type/style and
location around the stairwell area. Arbitrary swabbing was made around the DB
building of both the south and north stairwells. I, also, registered for the 11th
Annual Student Research Conference; I’m kind of nervous about how I have to
present a poster to professional educators. For instance, a vivid picture is
standing there with your poster, and many high ranked people poking at me with
their questions. Gilbert gave some insight on how their will be people like
that. I don’t really like to talk, but it is a weakness I can build on. I look
forward to it if accepted to their conference. A new experience will just
broaden my skills in speaking to the public. Moreover, I will just have to know
what I’m talking about.
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Figure 7. There is too much rails to grab when walking up and down stairs. |
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Figure 8. There was some nasty black stuff on the bottom of each rail. Everyone's fingers are touching it. |
Excellent posting!
ReplyDeleteD. Music