tag:blogger.com,1999:blog-30220949375717378012024-03-14T00:21:28.203-07:00S-STEM Scholar Darren Johnson's BlogAmanda Chapmanhttp://www.blogger.com/profile/16951554565276475785noreply@blogger.comBlogger36125tag:blogger.com,1999:blog-3022094937571737801.post-51904878264291840402014-03-06T15:48:00.001-08:002014-03-06T15:48:14.340-08:00Caution Failed Attempt<!--[if gte mso 9]><xml>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjB8kmju5XtZNGI_dRl7poC8_Spm0TnmFpuFvt13k7osf3bQRcLAkBx6RxykbHCcgiuPfEK1uBMspYtZNmRbTihsJqyCkc9BiD8DgkeMM24asg-z_VLqrSecJ0i466msSKLqu0IPn3Pdgr3/s1600/PICT0093.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjB8kmju5XtZNGI_dRl7poC8_Spm0TnmFpuFvt13k7osf3bQRcLAkBx6RxykbHCcgiuPfEK1uBMspYtZNmRbTihsJqyCkc9BiD8DgkeMM24asg-z_VLqrSecJ0i466msSKLqu0IPn3Pdgr3/s1600/PICT0093.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The duck tape around the rubber line.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg89iiQwXUwWPUfGj_EDfk2YXhFnNMtHwzYjklIxYVkXL53K3ACaDemwdaZBLpxjZUzkXDwVCjSE20FzcMWqxBJ4WHEsCjudD4PU_lGtp9b9Q1fPbXX77AJTNsww8_Pi5BJiaa1hAwdSvxO/s1600/PICT0092.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg89iiQwXUwWPUfGj_EDfk2YXhFnNMtHwzYjklIxYVkXL53K3ACaDemwdaZBLpxjZUzkXDwVCjSE20FzcMWqxBJ4WHEsCjudD4PU_lGtp9b9Q1fPbXX77AJTNsww8_Pi5BJiaa1hAwdSvxO/s1600/PICT0092.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. "Caution: The floor is wet!"</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Figure 3. The papers are all wet.</td></tr>
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I have worked on the gene gun this
week and had no success. But I only fall to get back up again and try. I was
missing some threaded tape to seal the threads between the metal for a more pressurized
seal. I was trying to reach a goal of 80psi but only got to 60psi before the
rubber line ruptured and leaked the air. I have modified the gene gun with some
duck tape because of my lack of finances in my project. I have also posted a
pic of a joke below the modified gene gun. Can anybody tell me if they get the
joke.</div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-51643389605866441472014-03-06T15:37:00.001-08:002014-03-06T15:37:49.071-08:00Dung Cannon<!--[if gte mso 9]><xml>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEix83IsaFaNY4_nT7yuA698i8DWLM3wSezobk3e2rwvABoftkXsyCo1VLRsUV_2R5aTHia_siOKubYzCaV_YJ8QwTK7lrsdGi9xJu3B5HJHiNKZ9CjxftR9SQTSnY8pu5HXClpE4wAZ_Grg/s1600/pilobolus_crystallinus_1.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEix83IsaFaNY4_nT7yuA698i8DWLM3wSezobk3e2rwvABoftkXsyCo1VLRsUV_2R5aTHia_siOKubYzCaV_YJ8QwTK7lrsdGi9xJu3B5HJHiNKZ9CjxftR9SQTSnY8pu5HXClpE4wAZ_Grg/s1600/pilobolus_crystallinus_1.jpg" height="197" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. This fungus is a close up of this 2-4cm tall stem with somewhat a black top hat full of spores. The vesical below fills with fluid creating a pressure to shootoff the black ball spore like object above.</td></tr>
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What
would most of my fellow inters think was the fastest accelerating object on
earth? A land animal like a cheetah, a hemi 357 engine with aftermarket add-ons
for better performance, a jet in the sky, or a fungus. The answer is a fungus
known as Pilobolus crystallinus. A fecal microorganism that cycles thru the animal
grazer intestinal system back out into fecal matter (feces) is part of their
life cycle. The question is how does this fungus get back to the mouth or entryway
of the digestion system? This 2-4 cm tall fungus shoots its spores about 6feet
away from its home of feces to a clearance where grazers eat. This 45mph shot
in one millimeter is the fastest accelerating object on earth. I have included
a video and a picture of the fungus below and above.</div>
<div class="separator" style="clear: both; text-align: center;">
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Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-60199305594490790132014-03-06T15:16:00.002-08:002014-03-06T15:17:53.151-08:00Species Education<!--[if gte mso 9]><xml>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhFpqfswA5P4KGgCzRyxPeh-TpOLitJ8bgWSvJE7vrtzyeJWDcuEK6fCYr2bmjy_9wfVu1jykFDaAzk8jCPXSC0RCwTshxAkIeU1y_uIuizm72rb5RCwS18EELmHGoT1CIP3WEHIzaym1Yj/s1600/cool.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhFpqfswA5P4KGgCzRyxPeh-TpOLitJ8bgWSvJE7vrtzyeJWDcuEK6fCYr2bmjy_9wfVu1jykFDaAzk8jCPXSC0RCwTshxAkIeU1y_uIuizm72rb5RCwS18EELmHGoT1CIP3WEHIzaym1Yj/s1600/cool.jpg" height="100" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1, 2, & 3. The pictures from the Wildlife World Zoo flamingo, aquarium with sea turtle (absent), and vulture. (from left to right, respectively.)</td></tr>
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<br /><iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='320' height='266' src='https://www.youtube.com/embed/l56K8eAtCig?feature=player_embedded' frameborder='0'></iframe></div>
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The trip to the Wildlife World Zoo
was a lot of fun from touching the stingray to their awesome aquarium. The view
of the animals was better than the Phoenix Zoo in my opinion. Although Amanda,
Josh, and me observed a vulture watching something move in the cluster of
leaves and shrub below its feet outside of its cage, we believed the mice were
diverting the attention away while another grab some its fruit inside the cage.
Moreover, I wished we had more time to experience the nursery they had. I look
forward to the next fieldtrip to the brewery. I have uploaded a video of a
snake devouring a crocodile. The video is graphic in some sense.</div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-68344380770777376672014-02-13T13:34:00.003-08:002014-02-13T13:34:46.553-08:00Simple Gene Gun<!--[if gte mso 9]><xml>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYMCdu8otyGwVFf4bWB5JYZrpZuaOtK6SiorXtKGEguZ7ttLvgD5yefuQTG6JwtCj7B-C5hVpw40zdLDRwWwOE7r8ymuw7YV7qhPiZ011wmj6LZq0cQmhHSpOWXzEIvu7qummY1Ke2fa7W/s1600/3Thrid_WeekBlogSpring2014.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYMCdu8otyGwVFf4bWB5JYZrpZuaOtK6SiorXtKGEguZ7ttLvgD5yefuQTG6JwtCj7B-C5hVpw40zdLDRwWwOE7r8ymuw7YV7qhPiZ011wmj6LZq0cQmhHSpOWXzEIvu7qummY1Ke2fa7W/s1600/3Thrid_WeekBlogSpring2014.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The blue box contain 13 mm in diameter disk filters placed atop of the pre-filter shown above in the lower left corner of the close up of a Swinnex filter. (Note: the disk filter 3 microns of material passing thru.)</td></tr>
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I worked on putting a gene gun
together this week with a 16-piece compressor set from Home Depot and a used
foot pump. The development of this very simple and inexpensive gene gun seems
it might work. On the other hand, I may have come across some problematic
obstacles heading down the road of this future apparatus. For instance, the Swinnex
chamber with the pre-filter in it has an inlet capacity of 50psi or 3.5bar. The
maximum pressure I will need for the successful acceleration of the tungsten particle
coated with the desired genes to shoot thru this filter is about 80psi or
5.5bar. Figure 1 above show the device separated into its individual pieces.
The red arrow indicates the flow of the air being compressed. I, also, added a
pic of the Swinnex filter in the lower right corner of Figure 1.</div>
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The attaching of the coupler and
other threaded areas needed Teflon tape for proper seal. I tried to use rubber
tape that was lying around in the robotics lab room. </div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-899929564124995152014-02-07T12:02:00.000-08:002014-02-07T12:02:16.896-08:00All This Reading for Nothing<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhV_3g0UedOjVG_aWUpJAvnzVh870jjzz4npihptYw-qOw0aVeb6Vw1oY71CKd2w_qPozzWuY0GGzqLgVGdD1DX2kJTIPY2REWRa-Q5IIiJeu6UY7FX7pJHNkYlusGNbGO_QEZNdIb0rCDl/s1600/cool+pic.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhV_3g0UedOjVG_aWUpJAvnzVh870jjzz4npihptYw-qOw0aVeb6Vw1oY71CKd2w_qPozzWuY0GGzqLgVGdD1DX2kJTIPY2REWRa-Q5IIiJeu6UY7FX7pJHNkYlusGNbGO_QEZNdIb0rCDl/s1600/cool+pic.jpg" height="383" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Diagram1. This poor diagram shows the mechanism of how helium used an force to push the particles of gold coated with the desired DNA in the syringe filter shoots into target of plant tissue. The purple oval shaped circles above the box is where the solenoid would go and the other two is the pressure gauges within the box.</td></tr>
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<div class="MsoNormal" style="text-indent: .5in;">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; color: #333300; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;">I started reading an article I found about
developing a simple gene gun apparatuses. It included welding pieces of 0.5
centimeters (cm) thick stainless steel sheets into a cube like shape. The open
side was covered with 1.5cm thick Plexiglas with 1.0cm thick rubber gasket
around the edges. The thick Plexiglas would serve as a door to the cube. Thus,
the overall specifications would have a solenoid attached to the top which it
served as the mechanism of the gun. Diagram 1 above shows the specifications of
the simple gene gun. The vacuum served to silent the nose of the compressed
helium shooting thru the shaft of the micro tube of the solenoid. The coated
gold particles with the desired genes are shot at a very high acceleration to
intervene with the nucleus of the plant cell without disrupting its normal
function. In conclusion, the desired DNA is inserted into the DNA of the plant
DNA if it was cut in some random area. This will overall make the DNA code for
some kind of gene expression. <o:p></o:p></span></span></div>
<br />
<div class="MsoNormal" style="text-indent: .5in;">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; color: #333300; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;">Josh on the other hand had a cheaper and less efficient
way of making a gene gun with a super soaker. Same concept but cheaper parts
and instead of gold for its particle to be coated it will be the chemical
powder tungsten. Also, the gas helium would be switched out for nitrogen and
carbon dioxide powder.</span></span><o:p></o:p></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-60220210645964329872014-02-04T11:25:00.002-08:002014-02-04T11:25:42.384-08:00I Can See Memory<div class="separator" style="clear: both; text-align: center;">
<iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='320' height='266' src='https://www.youtube.com/embed/SVhYF-ogjuM?feature=player_embedded' frameborder='0'></iframe></div>
<div class="MsoNormal">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; color: #333300; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;"><br /></span></span></div>
<div class="MsoNormal">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; color: #333300; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;">The process of
how memory is formed is visually caught in real time for a couple of seconds. The
mouse used as an in vivo experiment for this accomplishment had its mRNA or
messenger ribonucleic acid tagged with green fluorescent chemicals. Messenger
RNA encodes for the beta protein that shapes and structures the brain cell for
memory storage. The part of the brain known as the hippocampus was stimulated
to watch the mRNA go into process. The hippocampus role is separating events
and experience of short term memory to long term memory. Thus, driving a car for
the first time creates paths by the mRNA by encoding the nucleus of the neurons
to create molecules that shape the path of the dendrites synaptic communication
between other neurons. <o:p></o:p></span></span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjkG01tgFT2csHmsVMBq2nli7WvDie1xeNX-bIc3Vrti2rXJSjVLmRoTuxlQs-Xo_cIW7tuLCDJJBQ74MKasiD0gfebibCi7hnsT0yj9k-0LpT44Tu09TLqEmSWQnZff1y9QhTolDRGp4S7/s1600/Untitled.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjkG01tgFT2csHmsVMBq2nli7WvDie1xeNX-bIc3Vrti2rXJSjVLmRoTuxlQs-Xo_cIW7tuLCDJJBQ74MKasiD0gfebibCi7hnsT0yj9k-0LpT44Tu09TLqEmSWQnZff1y9QhTolDRGp4S7/s1600/Untitled.jpg" height="196" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The process of how the mouse mRNA was tagged with fluorescent green (A). The molecules forming for roles of creating memory of the neuron out to the dendrites (B, C).</td></tr>
</tbody></table>
<div class="MsoNormal">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; color: #333300; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;"><br /></span></span></div>
<div class="MsoNormal">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; color: #333300; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;"><br /></span></span></div>
<div class="MsoNormal">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; color: #333300; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;"><br /></span></span></div>
<br />
<div class="MsoNormal" style="margin-left: .5in; text-indent: -.5in;">
<span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;">Park et al. (2014). Visualization of dynamics of single
endogenous mRNA labeled in live mouse. Science. 343 (6169): 422-424. </span><span style="border: none windowtext 1.0pt; mso-border-alt: none windowtext 0in; padding: 0in;">doi: </span></span><span class="slug-doi"><span style="background: white; border: none windowtext 1.0pt; font-family: "Arial","sans-serif"; font-size: 9.0pt; line-height: 115%; mso-border-alt: none windowtext 0in; padding: 0in;">10.1126/science.123920</span>0<o:p></o:p></span></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-73643293931962766572013-11-27T11:41:00.001-08:002013-11-27T11:41:09.059-08:00Native Instincts Kicked In <div class="MsoNormal" style="text-indent: .5in;">
Well I can say I have a green
thumb. The pea plant seeds came out with all pots successfully growing into
sprouts. Figure 1 below shows the 100% success of all pots growing pea plants. Furthermore,
the green tray was split with my lab made divider using yellow tape and cotton
balls. I split the tray so the plants wouldn’t share the liquid of DI water and
faucet water, but it turned out that both liquids different in pH both grew the
plants. I think my last plants didn’t grow because the temperature was at 34
degrees Celsius; also, the soil was soaked rather than lightly moisten and the
mixed soil with vermiculite.<o:p></o:p></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgdWLqpDt1ETH_yaLs9sVv3MvCkylsNb2SeRpZPViT_5_aklZlFTlZFpMgclh8myB_z4cFhbTkRj08k0wULmM5IhGO3Nc2ipLe99XcxHJGkLaWlhh6WGyLUNg1Li5652GgMTo9ZVQyagyuS/s1600/Modified_PIC_Blog13.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgdWLqpDt1ETH_yaLs9sVv3MvCkylsNb2SeRpZPViT_5_aklZlFTlZFpMgclh8myB_z4cFhbTkRj08k0wULmM5IhGO3Nc2ipLe99XcxHJGkLaWlhh6WGyLUNg1Li5652GgMTo9ZVQyagyuS/s1600/Modified_PIC_Blog13.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The bottom left corner shows a close up of the success. There is a yellow arrow showing the yellow tape dividing the two rows of pots.</td></tr>
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I, also, sampled two locations off
campus for agrobacterium tumefaciens (A.tumefaciens). Figure 2 below show the
samples in 0.1% Peptone of 45mL with the corresponding test tubes. Once the sample
was chiseled out with sprayed 70% alcohol of a hammer, flat head screwdriver,
and chisel, it was immediately put into a urine sample cup of Peptone of 90mL
for isolation of bacteria. The piece of the crown gall was then rinsed with
sterile DI water then cut using a sterile scalpel and tweezers. This whole
procedure was performed in the flow hood for low risk of contamination. The pieces
cut were place in two sterile test tubes of 6mL of Peptone and two sterile test
tubes of 6mL of Tryptic Soy Broth (TSB). Then, the eight test tubes were stored
in a cabinet for room temperature of 25 degree Celsius for proper growth of
A.tumefaciens.<o:p></o:p></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiNZ3F7VTlizvq7mcd8CBjVbsm91-10bjDOOjNIkmGNZ4hgdl4HENT8d-nCczQGY0r2qClQNirltXCP5_1jt0hcts_xpC-_XazKdSFkeIxqa4VvAeAaomrJvpqCawnruRapX2PCf4etR7s-/s1600/Modified_PIC2_Blog13.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiNZ3F7VTlizvq7mcd8CBjVbsm91-10bjDOOjNIkmGNZ4hgdl4HENT8d-nCczQGY0r2qClQNirltXCP5_1jt0hcts_xpC-_XazKdSFkeIxqa4VvAeAaomrJvpqCawnruRapX2PCf4etR7s-/s1600/Modified_PIC2_Blog13.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The neon green top rows of test tubes are tree "A." The next 2 bottom yellow rows are tree "B." Basically, the top row has TSB. The next row has Peptone, then the next row is TSB. The final row has Peptone. </td></tr>
</tbody></table>
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<br /></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-18503767993126312542013-11-21T18:15:00.000-08:002013-11-21T18:15:14.428-08:00Interesting WEEK, <div class="MsoNormal" style="text-indent: .5in;">
In the beginning of this week I started
mixing my own soil for perfect growing conditions for my pea plants. The taxonomic
name would be Pisum sativum (P. sativum). These dicot plants would be a
good host for infection of the Agrobacterium tumefaciens (A. tumefaciens). Figure 1 below show the arrangement of the
seeds in the pots, and I used 70/30 of soil/vermiculite with a pH of 6.23. I was,
also surprised on how to calibrate a pH meter. Furthermore, Josh told me that
there was a pH meter for the soil that Matt had in his room. This meter gave a
reading of 7 and the calibrated meter gave a reading of 6.23. On the other
hand, the soil needed to be slightly acidic for growing conditions. Thus, the
top row would have only DI water and the bottom row would have regular faucet
water. I, also, trained newbie intern Alex on my procedure to prepare media and
sampling technique used to obtain bacteria on the made contact plates with TSA.<span id="goog_648516734"></span><span id="goog_648516735"></span><o:p></o:p><br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjFBTaxEzoDDPD6pWHX5TjSFd7bKKaTare2z06MXg-NQzf7Y45EplXP8VEeaeHYFn0IMS6gNocWNL27gJuX6jFTRN8ZmUGPelBXMuzloAulG5qghUcRhDqtukSOFnYbn660lLEUulGpqMAL/s1600/1.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjFBTaxEzoDDPD6pWHX5TjSFd7bKKaTare2z06MXg-NQzf7Y45EplXP8VEeaeHYFn0IMS6gNocWNL27gJuX6jFTRN8ZmUGPelBXMuzloAulG5qghUcRhDqtukSOFnYbn660lLEUulGpqMAL/s1600/1.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The pots are set up just the same way as this previous pic, but there is a divider in the middle so the water I put in don't mix.</td></tr>
</tbody></table>
<br /></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-36537871618072217602013-11-14T16:05:00.003-08:002013-11-14T16:05:58.389-08:00Failed Experiment<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh_-3u2EeSuL9CfesGItvCbq4KA50VjBgF9Bc8jSoWhyfrcQlwHtMOf-So9Qpd92DG6ESL2XNanuBLU2DisSpht6vivUGr85TAxaL3ndrV9BveiyoOMsPI6yW1SPFqydrpFrRzpRCrdl__K/s1600/Desk+10_07_2013+(1).jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh_-3u2EeSuL9CfesGItvCbq4KA50VjBgF9Bc8jSoWhyfrcQlwHtMOf-So9Qpd92DG6ESL2XNanuBLU2DisSpht6vivUGr85TAxaL3ndrV9BveiyoOMsPI6yW1SPFqydrpFrRzpRCrdl__K/s1600/Desk+10_07_2013+(1).jpg" height="320" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. This is just five of the twenty randomly used for observation and statistical analysis of the CFU. The rate of this group is fair.</td></tr>
</tbody></table>
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhB-xQXNBb0xvGmlfuyjNzNmu7-if7lb7XDm0sc426zZx19HOHCU9yyzzHXG9NfcXjVgbIvDsazeJtEaHxXfA-gpg43DKsgo5_WhwJS4vQED6mOEM3JyNKfftsEv_6UCBrvHGPpGU7jiYij/s1600/keyboard+10_07_2013+(1).jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhB-xQXNBb0xvGmlfuyjNzNmu7-if7lb7XDm0sc426zZx19HOHCU9yyzzHXG9NfcXjVgbIvDsazeJtEaHxXfA-gpg43DKsgo5_WhwJS4vQED6mOEM3JyNKfftsEv_6UCBrvHGPpGU7jiYij/s1600/keyboard+10_07_2013+(1).jpg" height="320" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The red obscure circular shaped colony in the top middle row is very interesting. The small white of a guess would be S. epidermis and the yellow medium sized circular would be S. aureus. The rate would be poor.</td></tr>
</tbody></table>
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEisXuiiQ5AE3EIxbqABWWyg528R-9rwTEZN0ceQ5OVehkbocZLQhyphenhyphenv5pqnUIU9dTbCHdYz2VGaHrGXg6FlwnCqZOEex-jVigQht35lzFsnpElTB4iNxawl2MY-Mz8a_D7U_-ntg2MzFVmOa/s1600/Mouse+library+10_07_2013.jpg++(1).jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEisXuiiQ5AE3EIxbqABWWyg528R-9rwTEZN0ceQ5OVehkbocZLQhyphenhyphenv5pqnUIU9dTbCHdYz2VGaHrGXg6FlwnCqZOEex-jVigQht35lzFsnpElTB4iNxawl2MY-Mz8a_D7U_-ntg2MzFVmOa/s1600/Mouse+library+10_07_2013.jpg++(1).jpg" height="320" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. I would say the rate for this group would be fair.</td></tr>
</tbody></table>
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgRmWQlrP1LZY42amf6vXnHpYg-pDijO5kz3xWiR7bRpeWPzlhp2sOAfA7I396OItqxoDq-f8Dzg3xj7sLrxH2pOquzX8oI2L3g7mKZHXDZZwKgKCo5wdBVi6DjqQf94wMyZRe6erVxrSBw/s1600/Rental+Bookstore+10_07_2013.jpg++(1).jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgRmWQlrP1LZY42amf6vXnHpYg-pDijO5kz3xWiR7bRpeWPzlhp2sOAfA7I396OItqxoDq-f8Dzg3xj7sLrxH2pOquzX8oI2L3g7mKZHXDZZwKgKCo5wdBVi6DjqQf94wMyZRe6erVxrSBw/s1600/Rental+Bookstore+10_07_2013.jpg++(1).jpg" height="320" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 4. </td></tr>
</tbody></table>
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjEfYWMooP3sf2BHqtH6diHzB3antqWSAgnB4T8TsWsn8FJdkY6NqCjSM4yDx5FhieK4zuYLwqpEshwA_Jud8Rm2P8WNidRykAnKCgqyI0KfKjpCeHIMMQxY5ua97WQcLL6seDDzMNqzIz8/s1600/Reserved+Bookstore+10_07_2013.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjEfYWMooP3sf2BHqtH6diHzB3antqWSAgnB4T8TsWsn8FJdkY6NqCjSM4yDx5FhieK4zuYLwqpEshwA_Jud8Rm2P8WNidRykAnKCgqyI0KfKjpCeHIMMQxY5ua97WQcLL6seDDzMNqzIz8/s1600/Reserved+Bookstore+10_07_2013.jpg" height="320" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 5. I would rate this group as very poor. There is a rigid circular shaped colony in the bottom row of the left plate.</td></tr>
</tbody></table>
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEihZXVPkUoyrWzeYwDr72_OwWryZQiq6ooy3qZlXyMp8yuoT_xDfX7fauXF-6-uyVd40-ki-LEaujbYX2umq7W06OLEFF81aKwSwEhg6EW9aLYZkzn8s1EwSJh5MceyGzy9LoihlitSX7_y/s1600/Seating+Area+10_07_2013+(1).jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEihZXVPkUoyrWzeYwDr72_OwWryZQiq6ooy3qZlXyMp8yuoT_xDfX7fauXF-6-uyVd40-ki-LEaujbYX2umq7W06OLEFF81aKwSwEhg6EW9aLYZkzn8s1EwSJh5MceyGzy9LoihlitSX7_y/s1600/Seating+Area+10_07_2013+(1).jpg" height="320" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 6. I would rate this group as poor.</td></tr>
</tbody></table>
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<br /></div>
<div class="MsoNormal" style="text-indent: .5in;">
The plants that I seeded with
Sonora wheat and Anasazi beans died because there was no watering due to my
absence of the lab. Although I infected three Anasazi beans with the possibly
A. tumefaciens, I wasn’t able to get any results because of my poor responsibilities
in the lab. On the other hand, my first project with the fomites is going
somewhat okay. I have sampled more than 8 locations around campus, and each
location had about 20 plates used for each site. If my calculations are
correct, I used about hundred and eighty plates, and trashed about a sleeve of
incorrect plates when pouring. The figures above show some of the randomly
obtained samples from each site that was going to be analyzed using Photoshop
software. Furthermore, I would rate the group as poor, fair, or acceptable for either necessary action or non-necessary action to be handled in the caption.<o:p></o:p></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-9376212660473159222013-11-07T14:10:00.001-08:002013-11-07T14:10:46.949-08:00Interesting Article<!--[if !mso]>
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I found a simple formula for how to calculate the sampling
sites of a location.<a href="http://www.microbiologynetwork.com/doc/Sutton.jgxp_.14.3.pdf" target="_blank"></a></div>
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<br /></div>
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<span style="mso-fareast-font-family: "Times New Roman"; mso-fareast-theme-font: minor-fareast;">Although the whole article was based on the
pharmaceutical industry of manufacturing healthcare products, the whole concept
of using contact plates is to make the sampling easier and quicker for my
project. The previous semester of using swabs and sterile tubes was a long
process from swabbing to inoculating to TSA media for incubation of CFU. It would
take about almost two hours just to sample an area with the swabs, but now the
contact plates make it easier to take samples. In other words, the PDF article
was beneficial on how to sample areas of potential contamination of
microorganisms. It, also, explains the proper procedures to take when analyzing
the results of the incubated plates after 24 hours. The simple equation above
used in a real situation would be used like the example below.</span></div>
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<span style="mso-fareast-font-family: "Times New Roman"; mso-fareast-theme-font: minor-fareast;"><span style="mso-spacerun: yes;"> </span>The
room used would be the DB-Building lab room 107 of the bottom floor.</span><br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgJSnrnM0zXO-m-TNN2qsO6hw56mO12LrHGqDk2XBiW7vxRSk-M_LRp1Rg-R8wLEc7cMgQQhgwo146zNk6x5cbYa0F4wksjiewTmMwZwSiEjdZnpf1tHALn4UR_5og5TPWAl9k-avZWCEVR/s1600/Untitled.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgJSnrnM0zXO-m-TNN2qsO6hw56mO12LrHGqDk2XBiW7vxRSk-M_LRp1Rg-R8wLEc7cMgQQhgwo146zNk6x5cbYa0F4wksjiewTmMwZwSiEjdZnpf1tHALn4UR_5og5TPWAl9k-avZWCEVR/s1600/Untitled.jpg" height="336" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. Shows the location of sampling to be taken.</td></tr>
</tbody></table>
<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgYE70UtgvNYMQTKMCFPoCETHajIZrheflY2g1_XsNvZa4qkwCRRJiFKcbWcMpSN0u0hMyxym8UdW5-m2_QbJ6bG32UbfovWTqBWC9dlzMeXRndvmeCPeAuaWlqk7HTAEGscjtoIrlRtJTS/s1600/Untitled22.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgYE70UtgvNYMQTKMCFPoCETHajIZrheflY2g1_XsNvZa4qkwCRRJiFKcbWcMpSN0u0hMyxym8UdW5-m2_QbJ6bG32UbfovWTqBWC9dlzMeXRndvmeCPeAuaWlqk7HTAEGscjtoIrlRtJTS/s1600/Untitled22.jpg" height="318" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. Shows the idea of how to calculate the minimum number of sampling locations.</td></tr>
</tbody></table>
<br />
<div class="MsoNormal" style="line-height: normal; margin-bottom: .0001pt; margin-bottom: 0in; mso-layout-grid-align: none; text-autospace: none;">
Where, <i style="mso-bidi-font-style: normal;">N<sub>L</sub></i> is the minimum number of
sampling locations (rounded up to a whole number)</div>
<div class="MsoNormal" style="line-height: normal; margin-bottom: .0001pt; margin-bottom: 0in; mso-layout-grid-align: none; text-autospace: none;">
A is the area of the
clean room or zone in meters<sup>2</sup>.<br />
<a href="http://www.microbiologynetwork.com/doc/Sutton.jgxp_.14.3.pdf" target="_blank">Article Link</a></div>
</div>
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<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;"></span><span style="mso-fareast-font-family: "Times New Roman"; mso-fareast-theme-font: minor-fareast;"></span></div>
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<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;"></span><span style="mso-fareast-font-family: "Times New Roman"; mso-fareast-theme-font: minor-fareast;"></span></div>
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<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;"></span><span style="mso-fareast-font-family: "Times New Roman"; mso-fareast-theme-font: minor-fareast;"></span></div>
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<span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;"></span><i style="mso-bidi-font-style: normal;"><span style="mso-fareast-font-family: "Times New Roman"; mso-fareast-theme-font: minor-fareast;"></span></i></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-82718538925525527232013-10-17T15:03:00.003-07:002013-10-17T15:03:39.070-07:00Fast Week with Progress<div class="MsoNormal">
The plants that were seeded last week didn’t germinate this
week, so the new approach was to use another type of soil known as vermiculite.
Vermiculite holds more moisture for less watering for the inexperienced
gardener. The green thumb isn’t in my DNA structure, but I still have six pots
of regular soil and another six of vermiculite. Also, the Sonora Wheat is still
being used but the soy beans were change to Anasazi Beans for germination.
Figure 1 below shows the arrangement of seeds planted.<o:p></o:p></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEioOcyUrYkPJwv78JqPJBJXKisbAKJHotTzczUcKQdLOyAYp3FnKrQAmf33fWJXUXxxNQqeyXKiFbfFBPuHuAz54dDvhWoe0ouAxnA7PA2hrZfXU8D71jT5L3ucvD7Mfn031mr3qO-TFTf6/s1600/PICT0049.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEioOcyUrYkPJwv78JqPJBJXKisbAKJHotTzczUcKQdLOyAYp3FnKrQAmf33fWJXUXxxNQqeyXKiFbfFBPuHuAz54dDvhWoe0ouAxnA7PA2hrZfXU8D71jT5L3ucvD7Mfn031mr3qO-TFTf6/s1600/PICT0049.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. I should start to see some sprouting next week by Monday at least if not then I'm a horrible planter. Furthermore, these seeds grow in places with less water like Arizona or other states along the Southwestern States.</td></tr>
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<div class="MsoNormal">
I haven’t sampled this week for my fomite project but I have
some potential ideas of where to sample at. The gym has some exercise equipment
that can be sampled, and maybe the smartphones students use around campus can
be sampled. I can prepare and pour TSA in the contact plates with no problems
now.<o:p></o:p></div>
<div class="MsoNormal">
<br /></div>
<br />
<div class="MsoNormal">
Furthermore, I took my sample from the residential property
across the street from the DB building. Figure 2 and 3 below show how I took my
sample with a couple of carpenter tools although the incremental borer would
make it easier. Once the sample arrived in the lab, a simple protocol was used
to isolate the potential specimen for Agrobacterium. On the other hand, the
results were not what I expected because of lack of ascetic techniques and
storing areas.<o:p></o:p></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5KxJXKmCInSN2hmu5SLKtCFTYwh84WdNZcJxAaC0HwznSmYTK-ZhWtvxAcnnLDFfrWqN1u8oKqpN4ROgK1Ny3H-wDBahtwm7KJkhHRddwVcsQmEv40GbccIpEEDJ6OkTkT-CBsYwVDWSW/s1600/PICT0044.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5KxJXKmCInSN2hmu5SLKtCFTYwh84WdNZcJxAaC0HwznSmYTK-ZhWtvxAcnnLDFfrWqN1u8oKqpN4ROgK1Ny3H-wDBahtwm7KJkhHRddwVcsQmEv40GbccIpEEDJ6OkTkT-CBsYwVDWSW/s1600/PICT0044.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. Approaching the area where the sample was taken is shown above. The inside volume is like a pyramid that is scraped out with tools like a flat head screwdriver, mini sludge hammer, box cutter, and some tweezers.</td></tr>
</tbody></table>
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhxrzLwPjcIII6H-3403JNGgflwgyOtpxiGQb-ahV_yK-m-53NISPgGgVDNaAjYxgN1AW_h7pscyU8rFzM92F16fxABVQ47ay3rvtO9Waes2AC37LGvIIJcaqnvTTQ0mPtYUXyvXm_GyCB_/s1600/PICT0048.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhxrzLwPjcIII6H-3403JNGgflwgyOtpxiGQb-ahV_yK-m-53NISPgGgVDNaAjYxgN1AW_h7pscyU8rFzM92F16fxABVQ47ay3rvtO9Waes2AC37LGvIIJcaqnvTTQ0mPtYUXyvXm_GyCB_/s1600/PICT0048.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. This side view shows the measured depth of the sample obtained on Wednesday October 16, 2013.</td></tr>
</tbody></table>
<div class="MsoNormal">
<br /></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-41866771771982591822013-10-08T16:03:00.002-07:002013-10-08T16:18:09.273-07:00Side Project<div class="MsoNormal">
The easiest way to perform a natural molecular biological transformation
with plants is using bacteria. The bacteria being used is Agrobacterium
tumefaciens (A. tumefaciens) that has a plasmid that can be easily transferred to
plant cells. Thus, this Gram-negative agent is used as a tool for engineering desired
genes into plants. The major cause of plant disease that this bacterium causes
is crown gall. Figure 1 and 2 below show some of the pictures I took around off
campus sites.<o:p></o:p></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5SEWTVFRzlpA7B79UEnl6PT-NjRO1ZZSENyiUg-y6fII-0ivCG2_62WcJT-kr_uGud3GKBUeRhTW3m6s9aGj_U4ChE-GD1UJ4YEZhLiWBW8odr5LRmotqQjy70eFvByFL3HLJLTNCKfTt/s1600/PICT0006.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg5SEWTVFRzlpA7B79UEnl6PT-NjRO1ZZSENyiUg-y6fII-0ivCG2_62WcJT-kr_uGud3GKBUeRhTW3m6s9aGj_U4ChE-GD1UJ4YEZhLiWBW8odr5LRmotqQjy70eFvByFL3HLJLTNCKfTt/s1600/PICT0006.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. This tree whole trunk is covered with crown gall. The tree is located just across the street from the DB Building at Phoenix College.</td></tr>
</tbody></table>
<br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg_5mWxrO_z17fjaunmTYuemCY8RtdnSBwSkiArA8KmmuJSrxciQJmGPPby1mxB6CDbp_LZfIGur8kTnB-ugjY8vbcUZxPR1NLIJ4U1y1KOzwyOkdVySOYzElsEb-lg7UINeWcbXuh8E5RP/s1600/PICT0010.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg_5mWxrO_z17fjaunmTYuemCY8RtdnSBwSkiArA8KmmuJSrxciQJmGPPby1mxB6CDbp_LZfIGur8kTnB-ugjY8vbcUZxPR1NLIJ4U1y1KOzwyOkdVySOYzElsEb-lg7UINeWcbXuh8E5RP/s1600/PICT0010.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The second site of this crown gall is located just a few more feet away near Thomas Rd.</td></tr>
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I started preparing my subjects below for germination and
the seeds used are soybeans and wheat. Figure 3 and 4 show the pots and
location of each per pot.<o:p></o:p></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhhm3NUZaGo2xEf6o9VXhGNxof9zKqNBujIFKhARXxw6AP6ILWHKjM4yGWytt4TR1Da5GSgQgq-aYM2i2gGVO52bsfvwFki7OQcUpF1vlx8Vuwq__DUHEN0km3zguPSmvIzYEH3n37tDMSL/s1600/Seeding_Layout.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhhm3NUZaGo2xEf6o9VXhGNxof9zKqNBujIFKhARXxw6AP6ILWHKjM4yGWytt4TR1Da5GSgQgq-aYM2i2gGVO52bsfvwFki7OQcUpF1vlx8Vuwq__DUHEN0km3zguPSmvIzYEH3n37tDMSL/s1600/Seeding_Layout.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. The seeds used are shown below the pots and the pots are numbered 1-6 just above the rim.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhpX2KCbP9FZ0Ly8RTPOc35RquaKesnG9gCynGv7L7tF9hXHlUWzdMLBzPMKeh1L0twzHKtRoE4kI_lrRsS8B72oaCZcOsz6Vl3FpXDejQjKJRFiLamVh_BsI9S5LCJcvbMLMZT_6CRAsHQ/s1600/Layout_of_Seeding.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhpX2KCbP9FZ0Ly8RTPOc35RquaKesnG9gCynGv7L7tF9hXHlUWzdMLBzPMKeh1L0twzHKtRoE4kI_lrRsS8B72oaCZcOsz6Vl3FpXDejQjKJRFiLamVh_BsI9S5LCJcvbMLMZT_6CRAsHQ/s1600/Layout_of_Seeding.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 4. The pots were placed in a 12 hour light incubator for growth around 1 o'clock on October 08, 2013. Note that the typo above in the picture is "seeds" for the "plants"</td></tr>
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I hope to identify these bacteria with positive growth of
the tumor once introduced to the plant with some type of identification marker
like pGLO added to the bacteria. I plan on thinking about how to introduce these
bacteria to the growing plants. For example, should I inject the disease
causing tumor in the stem, on the leaf, or on the roots? Should I mix with soil
or dip plant in solution of A. tumefaciens? Moreover, I look forward to a
positive Agrobacterium tumefaciens.<o:p></o:p></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-16944990861812696022013-10-04T12:31:00.003-07:002013-10-04T12:45:26.081-07:00Exciting, But Exhausting This Week<!--[if gte mso 9]><xml>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I sampled this week the school text books at the
bookstore and library of the campus. The used rental books at the bookstore of
Phoenix College were sampled on the cover. The picture below shows the titles
of each book sampled below.</span></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEie5Gzjz9bJEjz6_viNq_TnQwLUGoF37mrhj6eYYiTWUZU5SyD_9XgHEacmEEbD-Eoiwdj1qiKMAfTva24xmPPUHC-m4GH5wdIoCbo3DYZ6dSlLgUVFOO8IhSxl6NkanI7-EZf6rWXVxqcx/s1600/PICT0001A.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEie5Gzjz9bJEjz6_viNq_TnQwLUGoF37mrhj6eYYiTWUZU5SyD_9XgHEacmEEbD-Eoiwdj1qiKMAfTva24xmPPUHC-m4GH5wdIoCbo3DYZ6dSlLgUVFOO8IhSxl6NkanI7-EZf6rWXVxqcx/s1600/PICT0001A.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The numbers on the left side of the picture correspond with the plate sample. Some books were sampled twice on the covers.<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-bidi-language: AR-SA; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin;"></span><!--[if gte mso 9]><xml>
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<td class="xl63" style="border-left: none;"># CFU</td>
<td class="xl63" style="border-left: none;">Plate #</td>
<td class="xl63" style="border-left: none;"># CFU</td>
<td class="xl63" style="border-left: none;">Plate #</td>
<td class="xl63" style="border-left: none;"># CFU</td>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgnS_gbmcKeuJ1sXS2GvoYSWVCmAJu70Rf3kxHaqNHOmUKV9SpwNWVJkKWrnSyaeE4plWTtfiuzQlnPpEqRHCAgmTlWjD8I9tvplA3E4S4dFCRRjc1wjg5dmF0mXku2D2tqX3Oez8CLe1up/s1600/PICT0003.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgnS_gbmcKeuJ1sXS2GvoYSWVCmAJu70Rf3kxHaqNHOmUKV9SpwNWVJkKWrnSyaeE4plWTtfiuzQlnPpEqRHCAgmTlWjD8I9tvplA3E4S4dFCRRjc1wjg5dmF0mXku2D2tqX3Oez8CLe1up/s1600/PICT0003.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The books shown above are the reference books used throughout the school year if the student doesn't have enough money for the books in the campus bookstore or elsewhere. They can be checked out for 2 hours maximum if there isn't anyone else waiting for the book. </td></tr>
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<td class="xl65" style="border-left: none;"># CFU</td>
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<br />
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">The reserved books behind the counter of the campus
library were, also, sampled. The picture above show the books used in this
experiment.</span><br />
</div>
<div class="separator" style="clear: both; text-align: center;">
<iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='320' height='266' src='https://www.youtube.com/embed/MRBdbKFisgI?feature=player_embedded' frameborder='0'></iframe></div>
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<br /></div>
<div class="MsoNormal">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">Although I haven’t had much time in the lab this
week, I was pondering if it is possible to insert pGLO into plants for an illumination.
Basically, a plant that glows as it grows. For example, there are enzymes that
will break open an E.coli cell and combine the pGLO DNA into the ring DNA of
the cell for reproduction of glowing bacteria colonies. The process of
transferring exogenous DNA information and up taking it into another cell
membrane is called Transformation in molecular biology. The video above shows
how to conduct animal DNA transformation. The second video below shows how the start
of this project might begin with the first steps. Maybe?</span></div>
<div class="separator" style="clear: both; text-align: center;">
<iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='320' height='266' src='https://www.youtube.com/embed/Dc7QBPfX6sY?feature=player_embedded' frameborder='0'></iframe></div>
<div class="MsoNormal">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">for more information go to <a href="http://glowingplant.com/" target="_blank">Click this link</a> </span></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-57518676783322259992013-09-26T12:17:00.002-07:002013-09-26T12:50:03.731-07:00Excellent Week in LabThis whole week went as planned because I was right on schedule for sampling my objects around campus. Figure 1 below shows few places that was sampled in the Hannelly Center. The small side table tops attached to the chairs was sampled. The table below figure 1 shows the related colony forming units (CFU). <br />
<br />
<br />
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgdpIpTEeUyL7UH6goNulwESN2YzGH3w1ihlO02Lq6A6q4MvTTjVBHDEDcqOuzyK24J60hMD8rOloCa_rFkUsug0v_i07AHhej0dVHEJ1eWdDL8HC47l7ZUr2h0_djiacHjaIat4avOF5jd/s1600/PICT0910m.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgdpIpTEeUyL7UH6goNulwESN2YzGH3w1ihlO02Lq6A6q4MvTTjVBHDEDcqOuzyK24J60hMD8rOloCa_rFkUsug0v_i07AHhej0dVHEJ1eWdDL8HC47l7ZUr2h0_djiacHjaIat4avOF5jd/s1600/PICT0910m.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The red oval shaped areas are the general idea of where the sampling took place at. </td></tr>
</tbody></table>
Figure 2 and 3 below show the keyboard and mouse that was sampled in the library on the lower level of student resources for computer use.<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgisYbx5j6doHjG6Tazs5CSx4Q4LSH_HffxuRSKGGkEggWiCw5wt1fXCbBZXP8UazXzXpXoQCIE36c9Hi6SibXK6VhSc0eBN3funU0o6Qk4ZBijpsJnuVjMAY46w-wk8V_kt_3zv3Nzezzo/s1600/PICT0917.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgisYbx5j6doHjG6Tazs5CSx4Q4LSH_HffxuRSKGGkEggWiCw5wt1fXCbBZXP8UazXzXpXoQCIE36c9Hi6SibXK6VhSc0eBN3funU0o6Qk4ZBijpsJnuVjMAY46w-wk8V_kt_3zv3Nzezzo/s1600/PICT0917.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The original sampling was on the "space bar" but the area was too small for the contact plates, so each plate was firmly pressed on half of the contact plate and the above picture shows the idea of how it was sampled.</td></tr>
</tbody></table>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiCngV_00qld4HUAb5FtvABO8GZ6fpTSDLVZYZTH_cS1jPXnrI4CSzpQpxrxmsXQV1aZAk6VtS222CVnWu-tpNz_8tqd_RlLl_7RHn9OBNQBTsUhZZ2Ldwqkb4BM88h9sHyTUzm2_g2Wvsk/s1600/PICT0913.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiCngV_00qld4HUAb5FtvABO8GZ6fpTSDLVZYZTH_cS1jPXnrI4CSzpQpxrxmsXQV1aZAk6VtS222CVnWu-tpNz_8tqd_RlLl_7RHn9OBNQBTsUhZZ2Ldwqkb4BM88h9sHyTUzm2_g2Wvsk/s1600/PICT0913.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. Some mouse were sample twice and some where sample just once on the top with a rocking motion. If another plate was to be sampled on the same mouse, then half of it was sampled on the sides of the mouse.</td></tr>
</tbody></table>
The Bear Cave Cafeteria was, also, sampled around dinning area. Furthermore, the table tops were observed by myself of a cashier wiping down the tops with a rag. Thus, the colony plate count should be low, but I believe the microorganisms were just moved around.<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhLbP4bNDxw66S_2sCs-T4uIEOCzpuKieLNH9UUKOh97yM8Wqwm4vEB23YIjwF9y_CDX6yIeuDvT32hnrFAQHBIrqXGmQ6QEZdEL3yPuIC1c29jKMn7VFURs_j77peC0wWs-J18ENfgf0V8/s1600/PICT0919.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhLbP4bNDxw66S_2sCs-T4uIEOCzpuKieLNH9UUKOh97yM8Wqwm4vEB23YIjwF9y_CDX6yIeuDvT32hnrFAQHBIrqXGmQ6QEZdEL3yPuIC1c29jKMn7VFURs_j77peC0wWs-J18ENfgf0V8/s1600/PICT0919.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 4. The area of eating in the cafeteria.</td></tr>
</tbody></table>
<br />Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-1587558171781987342013-09-19T16:26:00.000-07:002013-09-19T16:26:04.469-07:00Organized and Ready<!--[if gte mso 9]><xml>
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I started this week off with a
great start. I have a good amount of contact plates with no problems of loss of
inventory. I prepared about six sleeves of 20 plates this week. Figure 1 below
shows the progress of perfect dome shaped above the rim of the plates being
prepared. I modified the dispensing of 17mL to 16mL because of the overflow and loss of plates.</div>
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<tr><td class="tr-caption" style="text-align: center;">Figure 1. The plates are being UV for further sterilization.</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Figure 2. The numbers correspond to the table 1 below of total Colony Forming Units.</td></tr>
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<td class="xl65" height="20" style="border-top: none; height: 15.0pt;">1</td>
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<td class="xl65" style="border-left: none; border-top: none;">6</td>
<td class="xl65" style="border-left: none; border-top: none;">82</td>
<td class="xl65" style="border-left: none; border-top: none;">11</td>
<td class="xl65" style="border-left: none; border-top: none;">54</td>
<td class="xl65" style="border-left: none; border-top: none;">16</td>
<td class="xl65" style="border-left: none; border-top: none;">19</td>
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<td class="xl65" style="border-left: none; border-top: none;">7</td>
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<td class="xl65" style="border-left: none; border-top: none;">12</td>
<td class="xl65" style="border-left: none; border-top: none;">47</td>
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<td class="xl65" style="border-left: none; border-top: none;">8</td>
<td class="xl65" style="border-left: none; border-top: none;">65</td>
<td class="xl65" style="border-left: none; border-top: none;">13</td>
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<td class="xl65" style="border-left: none; border-top: none;">18</td>
<td class="xl65" style="border-left: none; border-top: none;">29</td>
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<td class="xl65" style="border-left: none; border-top: none;">9</td>
<td class="xl65" style="border-left: none; border-top: none;">62</td>
<td class="xl65" style="border-left: none; border-top: none;">14</td>
<td class="xl65" style="border-left: none; border-top: none;">26</td>
<td class="xl65" style="border-left: none; border-top: none;">19</td>
<td class="xl65" style="border-left: none; border-top: none;">12</td>
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<td class="xl65" style="border-left: none; border-top: none;">30</td>
<td class="xl65" style="border-left: none; border-top: none;">10</td>
<td class="xl65" style="border-left: none; border-top: none;">39</td>
<td class="xl65" style="border-left: none; border-top: none;">15</td>
<td class="xl65" style="border-left: none; border-top: none;">18</td>
<td class="xl65" style="border-left: none; border-top: none;">20</td>
<td class="xl65" style="border-left: none; border-top: none;">35</td>
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I, also, sampled the plates on two
locations around campus. The first location was room 221 of the DB building on
the second floor. Figure 2 above shows the area being monitored for fomites and
the corresponding plates to CFU’s. I noticed that as you move from the back to the front of the room the CFU's get smaller per plate. </div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-48776294335762730902013-09-11T16:21:00.001-07:002013-09-11T16:21:05.720-07:00Lesson Learned<!--[if gte mso 9]><xml>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I started to pour the
agar using a Multipipette Plus with a 50 mL syringe for a total volume of 17 mL
added to each empty contact plate. I did some research on the plates and found
there name to be known as RODAC (</span>Replicate Organism Detection and
Counting) <span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">plates.
The supplying company recommended filling the plates on their website between
16.5-17.5 mL for proper convex surface to be achieved. The figure below shows
some of my flaws conducted Monday in the flow hood. </span></div>
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<span style="font-family: "Times New Roman","serif"; font-size: 12pt; line-height: 115%;"> <table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhiWluF9iWYR_31U8CktpmPmuHGWsOEvzuzZY7Qo7tuou545KDm6D6f2mSTOIfRVK7E9MXiMCNow3xuApVy-F3pRxJhPaHG-K-Fziqyd3P4HtBJJRfVV6zTFq5vNWuFrEmXXXGXS8HTsTzA/s1600/PICT0898.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhiWluF9iWYR_31U8CktpmPmuHGWsOEvzuzZY7Qo7tuou545KDm6D6f2mSTOIfRVK7E9MXiMCNow3xuApVy-F3pRxJhPaHG-K-Fziqyd3P4HtBJJRfVV6zTFq5vNWuFrEmXXXGXS8HTsTzA/s1600/PICT0898.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The spill was so quick and sensitive to touch that the cohesion of any surface quickly pulled it over the lip or ring around the top.</td></tr>
</tbody></table>
</span></div>
<br />
<br />
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiB_ujBZfjRPZKqvM9XbGaAGxd4ZSiURijBAg5T1TxuMX6BT-mkqwd5yT4em_W-l3mTO6DGS8u2nSh8eUA5OJaXfLdDz0ApiY45rA7sHjdT0Eo4g4ZF_Wcqesmns9mrk0sYPBVQJq6UMJco/s1600/PICT0900.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiB_ujBZfjRPZKqvM9XbGaAGxd4ZSiURijBAg5T1TxuMX6BT-mkqwd5yT4em_W-l3mTO6DGS8u2nSh8eUA5OJaXfLdDz0ApiY45rA7sHjdT0Eo4g4ZF_Wcqesmns9mrk0sYPBVQJq6UMJco/s1600/PICT0900.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The plates were left in the hood for 10 minutes with the ultraviolet light on for proper sterilization.</td></tr>
</tbody></table>
<br />
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<span style="font-family: "Times New Roman","serif"; font-size: 12pt; line-height: 115%;"> </span></div>
<div class="MsoNormal" style="text-indent: 0.5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12pt; line-height: 115%;">The contact of the lid and
liquid caused multiple spillage and contamination. The first thing I need to
work on is conserving the empty plates by making no mistakes for contamination.
Thus, there will be no waste of agar and RODAC plate inventory. Furthermore, I need
to be more quick, efficient, and organized. I, also, noticed on Monday that the
plates need to cool without the lids. Dispensing the warm TSA liquid from the
pipette needs to be quick in regards to time because of the solidification of
the Multipipette syringe.</span></div>
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<br /></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi1f6Ii8bMNNoqvk13u48NBomWUnjyTR6lWaikOVvBapxgqkgFAw2jxwKTa2zaQR7apeYhn2M0zmrPPafKQrxrQfl-Z7BvdTfCKjrLsLlSgYrJxORuy1L0jNU08tFnbnYxbLdUMUmgMaCS2/s1600/PICT0899.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi1f6Ii8bMNNoqvk13u48NBomWUnjyTR6lWaikOVvBapxgqkgFAw2jxwKTa2zaQR7apeYhn2M0zmrPPafKQrxrQfl-Z7BvdTfCKjrLsLlSgYrJxORuy1L0jNU08tFnbnYxbLdUMUmgMaCS2/s1600/PICT0899.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. This shows the muffin top like shape of the solidified tops of these contact plates.</td></tr>
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<br /></div>
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<br /></div>
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<br /></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-89871650575913669622013-09-06T12:59:00.003-07:002013-09-06T12:59:52.981-07:00Late Start<div class="separator" style="clear: both; text-align: left;">
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<![endif]--><span style="font-family: "Calibri","sans-serif"; font-size: 11.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin;">I
started my hours for the internship this week on Friday. On the other hand, I decided
to continue my project with an easier step of obtaining samples. I will prepare
my own agar for pouring into contact plates which will eliminate the hassle of
the “swabbing method.” The contact plates are miniature plates with a molded
grid on the bottom of this bulb like surface. I plan to prepare and pour
the agar after the thirty minute water bath from the autoclave. Figure 1 below shows three
empty contact plates and the Pipet used during the filling of these plates. </span></div>
<div class="separator" style="clear: both; text-align: left;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11pt; line-height: 115%;"> <table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjnqoJvWkr7AtWLFJQSSdmTLEHaSK2Xke1b20Kxyox7PYUJKS3s3rz-nkUsb8eslbmQJ-2C2EZRKUYkD04Duzaa-sdr-3Cub23KnTZirxk-pltgAq4hPXVH4ZporLlf0-GMbcTO0ij6GIaI/s1600/PICT0895.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjnqoJvWkr7AtWLFJQSSdmTLEHaSK2Xke1b20Kxyox7PYUJKS3s3rz-nkUsb8eslbmQJ-2C2EZRKUYkD04Duzaa-sdr-3Cub23KnTZirxk-pltgAq4hPXVH4ZporLlf0-GMbcTO0ij6GIaI/s1600/PICT0895.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The bottom isn't flat like other plates, but it has like a bulb like surface. The Pipet is used for multiple dispensing of efficient volume because the maximum volume is 17 ml.</td></tr>
</tbody></table>
</span></div>
<div class="separator" style="clear: both; text-align: left;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11pt; line-height: 115%;"> <table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiDiuegShZPv8-jyEn7Xtg1pVTVeY38LD8PnqEeVlOLbaVYhHwpjJbEdKO68gWK6LpZfJtBJ2e47QhKPUmfbP5ggCxnXD9hZDBswJnVhZMxMIiZkB-Sa3-ooiEIy8uib8ldDVOyVSIPwQkq/s1600/contact-plates.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiDiuegShZPv8-jyEn7Xtg1pVTVeY38LD8PnqEeVlOLbaVYhHwpjJbEdKO68gWK6LpZfJtBJ2e47QhKPUmfbP5ggCxnXD9hZDBswJnVhZMxMIiZkB-Sa3-ooiEIy8uib8ldDVOyVSIPwQkq/s1600/contact-plates.jpg" height="265" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The agar is slightly above the rim of the plate for making contact with surfaces of objects.</td></tr>
</tbody></table>
</span></div>
<div class="separator" style="clear: both; text-align: left;">
<span style="font-family: "Calibri","sans-serif"; font-size: 11pt; line-height: 115%;">Figure 2 shows the finished product of an elevated agar to make direct
contact with surfaces around campus. I was going to use the dispenser machine,
but the mentors noted that the dispenser is outdated. I look forward to next
week. I, also, took a picture of the dispensing machine below in Figure 3.</span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg_FyKDgshPTq5Ab4YTOa9RXKZS1IBddELqGmSDDRZ2KnxNd0PuEnOSsXz2PtYE6n-xgGHOk7tnjowwK9Y0NMmIQ5CimgaJ9Ue7vIUqd7W6lCBQB3YO-B5V1-kpIxod8nnTw4ziTYNZM9qu/s1600/PICT0896.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg_FyKDgshPTq5Ab4YTOa9RXKZS1IBddELqGmSDDRZ2KnxNd0PuEnOSsXz2PtYE6n-xgGHOk7tnjowwK9Y0NMmIQ5CimgaJ9Ue7vIUqd7W6lCBQB3YO-B5V1-kpIxod8nnTw4ziTYNZM9qu/s1600/PICT0896.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. I always saw this machine but never new what it did until today.</td></tr>
</tbody></table>
<div class="separator" style="clear: both; text-align: left;">
<br /></div>
<br />Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-48753121743494394912013-05-02T21:42:00.001-07:002013-05-02T21:42:17.599-07:00Take Flight<div class="separator" style="clear: both; text-align: center;">
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Although I have no time to join the robotics club, I found a
cool article about a tiny robot as small as a quarter hovering the way actual
insects do. Weighing under 80mg, it is now being conducted to try some ways of
finding a power source light enough to hold. If the robot has artificial
material to mimic the wings and muscle of a real insect, then the invention of
micro robotics is in a near future in my opinion. I have linked the article
below and attached a video above for further reading</div>
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<br /></div>
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<a href="http://www.nature.com/news/tiny-robot-flies-like-a-fly-1.12926">http://www.nature.com/news/tiny-robot-flies-like-a-fly-1.12926</a></div>
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<br /></div>
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I finished a seven page report, and need to modify my final
presentation poster by tomorrow. Although the outlook looks excellent in my
favor, I hope to finish tonight and post it to Dropbox by tonight. The semester
looks good to the current grades I have been getting. The grades look like 5 “A’s”
and 2 “C’s.” the C’s come from my view as sacrificed courses until I get back
on schedule. I wanted to sample the cafeteria and library computers before I submitted
the poster, but it looks like I’m going to have to settle with what I got. There
hasn’t been much I did instead of preparing for the project paper and poster.</div>
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<br /></div>
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Since I haven’t identified any of the microorganisms in my
project, I have noticed that the colonies I’m counting can just be resident
flora of the hands. The men’s bathroom, for example, may have more of an
average CFU count to the women’s bathroom, but the colonies observed can just
be resident flora microbes. I haven’t identified if the colonies observed were
coliforms/enteric bacteria that are picked up and spread around the campus.
Staphylococcus species like S. epidermis live on our skin. Even though you wash
your hands, the amount of epidermis will never completely come off. Scrubbing with
a brush will just spread them all over your fingers and palms. The only removal
of bacteria is transient bacteria. Transient bacteria are the microbes picked
up in the bathroom or other places of contact. Thus, I should in my modified
project identify the microbes being sampled. </div>
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The project gave me insight that microorganisms are
everywhere, and the hands are the main transmission of contacting a disease
causing bacteria. The individual with a good immune response is the reason for
being healthy every day.</div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-70598737609094889802013-04-25T20:01:00.002-07:002013-04-25T20:01:44.400-07:00Final Exam Week Approaches<!--[if gte mso 9]><xml>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">The spring semester is coming to an end, and I realize
that taking too many classes doesn’t work for me. One reason was that I didn’t
get to spend that much time with my kids this part of the year. For example,
the only time I saw my oldest son was at night when he was sleeping or in the
morning heading off to school. I spent most of my time at school, I felt like I
ate, slept, and worked at the campus. Although it did payoff in the end, the
grades I got were acceptable before the final exam results. Good Luck to all
fellow interns for exam week. I look forward to next semester in the fall, and
plan to work on a car to broader my knowledge in repairing things like Fix-It-Felix,
Jr.</span></div>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;"> <table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYIcZkekrCp5YVEKv9zGVGz9v_FMTvdyCuQRwCkIolbpEQoWWjzwRUDc5ruJYeMiDF2GNk411kjgwUY7lCueI9-nmWFoqqsfAeFzhL3DZE3FE2bZ9VoxL-dh2q0cYM9EQrBrYcXPqlT1vj/s1600/tests.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiYIcZkekrCp5YVEKv9zGVGz9v_FMTvdyCuQRwCkIolbpEQoWWjzwRUDc5ruJYeMiDF2GNk411kjgwUY7lCueI9-nmWFoqqsfAeFzhL3DZE3FE2bZ9VoxL-dh2q0cYM9EQrBrYcXPqlT1vj/s1600/tests.jpg" height="266" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The picture shows the Scantron form in action for a exam. Again good luck to the lab interns who have final exams.</td></tr>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">On the other hand, my knowledge around the bio department
is more of a natural reaction. For instance, I can walk into the lab and mix,
autoclave, and pour media; moreover, I can prepare sterilize test tubes, sample
and inoculate plates, and count CFU’s if I have previous incubated plates from
the previous days of sampling. It seems like a lot in one day, but it is a natural
outcome due to the hours put into the internship. If I had time to do something
else after the project, I would autoclave bio waste of the biohazard bags, help
Kim setup labs or disassemble labs, wash dishes, or other tedious work. I know I
used the word “tedious,” but I meant tiresome and not boring like the
definition. The constant pace of pouring three hundred plates of TSA liquid
would be the meaning of the word “tedious” in my sentence above</span></div>
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<br /></div>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I have sampled this week the Handicap buttons, lab
work benches, and the classroom Apple IPads. I thought the handicap buttons
would have more CFU’s, but the result showed that the inside bathroom buttons
were mostly contaminated. The sun beaming down on the campus could be another
reason for low counts on the handicap buttons. The UV rays can disrupt their
DNA which it codes for their function as a microbe. Furthermore, the temperature
for the week of Phoenix, AZ has been an average of 93°F for the past three
days. Thus, the temperature converted to Celsius is 33.3° C. In conclusion,
bacteria want moisture, warmth, and nutrients; they existed more in the
bathroom because the moisture from maybe completely dried hands and locked in
moisture from the outside temperature. The air outside is dry and the sun
hitting the handicap door maybe messed with the results. The updated bar graph below in Figure 2 show them in order from highest to lowest.</span></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjO8K_IpGKGjCzGdLcafYBQXkRY0luBc-bYLRx17pFf5V-0m8QBg8_O_N5uuOAFZerZSH5kfXHJnRZYFaFCwqoExn9IJKzudHkqQbVvwiqzvlWbsH95cH9Bdp1568Q4VpXIlFr31vGfRsz9/s1600/Untitled.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjO8K_IpGKGjCzGdLcafYBQXkRY0luBc-bYLRx17pFf5V-0m8QBg8_O_N5uuOAFZerZSH5kfXHJnRZYFaFCwqoExn9IJKzudHkqQbVvwiqzvlWbsH95cH9Bdp1568Q4VpXIlFr31vGfRsz9/s1600/Untitled.jpg" height="263" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. It looks like the lab workbenches of the DB building are less in CFU's. Thus, the highest is still drinking fountains, and soap dispensers came in second. In conclusion, outside objects in Arizona seem like they pose likely no harm for contacting or gaining transient flora on the hands.</td></tr>
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Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-33081172192821185272013-04-18T21:59:00.004-07:002013-04-18T21:59:43.714-07:00Don't Kill My Vibe<!--[if gte mso 9]><xml>
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<span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%;">I found a useful website known as Khan Academy, and it
has thousands of sorted videos for students. For instance, I needed a quick
review of chapters in organic chemistry and this site had videos in order of
concepts, and it was easy to navigate. I hope this information is useful to
other students. In other words, I think visual learners like me would find this
tip really helpful other than reading a whole chapter before a quiz or test. Furthermore,
the videos are no longer than 12 minutes. There is no catch or some type of fee
to pay, so just hit the “Learn” link to open more options.</span></div>
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<br /></div>
<div class="MsoNormal" style="text-align: center;">
<span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%;"><span style="mso-tab-count: 1;"></span><a href="https://www.khanacademy.org/">https://www.khanacademy.org/</a></span></div>
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<br /></div>
<div class="MsoNormal">
<span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%;"><span style="mso-tab-count: 1;"> </span>The soap
dispenser in the bathrooms was sampled in a total of forty. I, also, recorded
in my journal what genders was being sampled from. Luckily, I had an even split
of twenty women and twenty men. Thus, I made a graph of the dirtiest soap
dispensers of men vs. women. Maybe somebody in the lab can pursue this project
of soap dispensers between men and women. Overall, the soap dispenser took
second place of the average CFU’s with drinking fountains still being first. I
sampled this week the door handle of bathrooms around campus, and they came in
second. Bathroom door handles knocked soap dispenser beneath it. Figure 1 shows
the updated graph of fomites. Figure 2 shows the soap dispensers between men
and women.</span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhq8lzptEXCfeBShxJ7tLcMGTPswrWssIHsH7cFoOWCHBtXEAsT8FwYcWCmKcW0AJHYAEJIyFllTdAPFgq777Ena5bph_EPnbHX39QotSSC_iW_DzisqEjDG2wY0GOwxkenqzAv2oZfcPqL/s1600/Untitled.png" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhq8lzptEXCfeBShxJ7tLcMGTPswrWssIHsH7cFoOWCHBtXEAsT8FwYcWCmKcW0AJHYAEJIyFllTdAPFgq777Ena5bph_EPnbHX39QotSSC_iW_DzisqEjDG2wY0GOwxkenqzAv2oZfcPqL/s1600/Untitled.png" height="262" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The standard deviation seems high on both the drinking fountain and bathroom door handles. I still look forward to adding more place or objects to sample in the future.</td></tr>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhBZ_5j10SAjBgMTkM55ZNxCPAe78ak1SCCXoHxT9F0NQC78LA-FoIX5Mfg-kWfuc57bKjiUm8ZJlsACO-gx-_eeOkQSQmZIcPBT07PGXinwkNqPyBgqZkSCFXI3peqsl9LeCWxrmX4sQdl/s1600/Untitled.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhBZ_5j10SAjBgMTkM55ZNxCPAe78ak1SCCXoHxT9F0NQC78LA-FoIX5Mfg-kWfuc57bKjiUm8ZJlsACO-gx-_eeOkQSQmZIcPBT07PGXinwkNqPyBgqZkSCFXI3peqsl9LeCWxrmX4sQdl/s1600/Untitled.jpg" height="262" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The men in the blue seem just slightly above the women in the red. The button to dispense the foamy soap was sampled and nothing else.</td></tr>
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<![endif]--><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-bidi-language: AR-SA; mso-fareast-font-family: Calibri; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-latin;"> I notice that maybe men are just a little more covered in microbes than
women. I notice in one of my microbiology labs that since I’m just sampling
without identifying the colonies. It can be that the colonies I grew were
resident flora bacteria of the hands. Thus, the removal of transient flora
bacteria can be washed off, but the resident flora will just reduce the number
of bacteria. The most prevalent bacteria on human skin are Staphylococcus
epidermis. This could be an explanation of the colonies growing on the
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<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiegVpUgKMACV0xDFsYSqoDHv8p6QD0ZWquViq3ftiC5kyN3503fbUs-c5XvM-TI7pQRkt5Pb7lODkFndypjmvyGoVOYtgawOV_fHdq4GT4R07SQcD7H0gTWPolYjDFUwIOPJ7Uuk3bv-_B/s1600/Untitled.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiegVpUgKMACV0xDFsYSqoDHv8p6QD0ZWquViq3ftiC5kyN3503fbUs-c5XvM-TI7pQRkt5Pb7lODkFndypjmvyGoVOYtgawOV_fHdq4GT4R07SQcD7H0gTWPolYjDFUwIOPJ7Uuk3bv-_B/s1600/Untitled.jpg" height="262" width="400" /></a></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 12pt; line-height: 115%;"><span> </span>I, also,
compared the classroom desk of buildings. Figure 3 above show the comparison
between mostly all the highly used buildings holding classrooms. Furthermore, I
have noticed that after one class another class will be rushed in on some
circumstances. Some students bring sanitizing wipes like Lysol, but they only
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only gram positive microbes and not gram negatives or coliforms.</span></div>
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<br /></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 12pt; line-height: 115%;"></span></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-35772344349837406972013-04-15T17:39:00.002-07:002013-04-15T17:39:35.916-07:00Please Excuse My Absence<!--[if gte mso 9]><xml>
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I had been sampling and counting
plates, since I came in for the first week of this month, but it came to a halt
last week when I missed the whole week. It was like a daily routine before my
absences in the lab. A tedious workload that has its benefits is considered the
duty of student interns.</div>
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<br /></div>
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<span style="mso-spacerun: yes;"> </span><span style="mso-spacerun: yes;"> </span>I,
furthermore, notice that one of my flaws in the Bio department (dept.) is
pouring plates for TSA media. For instance, Josh, Jose, and I poured plates and
took one solidified plate from each batch that each person had made and let it
sit above the window niche. The next days showed that I had colonies of growth,
and Jose was second for growth. I believe that my plate had growth in only one
day, and Jose was about 2-3 days of growth. Matt asked, “If I was talking or
taking breaks from pouring?” and this gave the idea that I should start pouring
in the filtered hood. In other words, I lack the skill of pouring media. The video
below shows how to simply pour media in the lab, but I don’t believe we drop
antibiotics or x-gal into the hot liquid.</div>
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<div class="separator" style="clear: both; text-align: center;">
<iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='320' height='266' src='https://www.youtube.com/embed/rEMfRXr3wpc?feature=player_embedded' frameborder='0'></iframe> </div>
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<br /></div>
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The most exciting target for those
weeks was soap dispenser buttons in the bathrooms. Also, the desk that student
sit at during lecture was sampled. I walked around the campus of the English
dept., math dept., and chemistry dept. for sampling. I would have to admit I
saw a few instructors from the bio dept. all over campus like Rosati and other
instructors who know of my project. I’m, basically, pushing a cart around with
a miniature orange biohazard trash container, sterile swabs, and test tubes. I
still get stares like in the elevators and other places where I’m swabbing, but
I ensure that I’m pursuing a project or experiment.</div>
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<br /></div>
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Unfortunately, I wasn’t accepted to
the Estrella Mountain Conference in May. The second week of this month was a
total failure for my internship because I was absent throughout the week. I
did, however, attended my scheduled classes to keep up with my grades. I have
updated my graph of my project and it looks like the soap dispensers were,
also, high in average colony counts.</div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgyUzfSHSs3q0pFugyqDcn1QXNrr6eBbQpC_6bqAydYM1Q3eik8xJGQ4BaT3VbBzqSw9XwbIGboSps0J5sqWfUmrfHuSThKSSY2t1WwiIqhEmOosr9VCFBvcoNv2di-PqRRWbveAGMwGe7h/s1600/Untitled.png" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgyUzfSHSs3q0pFugyqDcn1QXNrr6eBbQpC_6bqAydYM1Q3eik8xJGQ4BaT3VbBzqSw9XwbIGboSps0J5sqWfUmrfHuSThKSSY2t1WwiIqhEmOosr9VCFBvcoNv2di-PqRRWbveAGMwGe7h/s1600/Untitled.png" height="262" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The graph needs to have more bars of objects, and it will look more professional. Hopefully, I will have more places to sample.</td></tr>
</tbody></table>
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I read from an online source that this more
traditional dispenser is preferred because of its easy foaming germ-killing
power. It, also, dispenses 1,700 times. Thus, it can serve a range from 300-850
people depending on the number of dispensing during hand washing techniques.
This is a huge area of transferring microbes because you would think that the soap
from hand contact and water would lower the count in that area. My educated
guess is that dirty hands with either resident flora or transient flora make
contact with the button, and no further cleaning techniques are pursued on the buttons
around campus. Thus, the accumulation of microbes occur with more transfers.</div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-47442453648277496442013-03-28T15:14:00.000-07:002013-03-28T15:14:20.065-07:00Lab Technician Wisdom<!--[if gte mso 9]><xml>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">The project is
currently being modified. First, the samples have increased from twenty to
forty of objects containing fomites. I have increased the sampling because the
error bars become extremely high in my past bar graphs of my standard
deviation. My first attempt to correct error bars is as follows: I got rid of
my outliers or outside values of my set of data, but half my data was eliminated
or taken out from my set of data. The removal of these values caused me to have
a total of 12 or less values, and the total sampling was 20. In conclusion,
twelve divided by twenty will give me 60% of data and 40% of lost data.
Furthermore, increasing the sampling will have less removal of outliers and
more values for better averaging. Thus, the average values of my data or
quantified results have been better, and also my standard deviation has better
results. Figure 1 below shows how I came to this conclusion of increasing my
sampling in a small range of data.</span></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh5EKygd4gxuF5bylAEZOgBf8WgW7B1Ctpd7OCmMNa2pgvCItIAogicScb7n7DLAoYuih6yvQ896jZYC62S3ahAAcCXpcK02HLowoS9yar0Y-Ke9EWP8cO8zADYPFxdsrK7gkxO6HVWTun5/s1600/ideas.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh5EKygd4gxuF5bylAEZOgBf8WgW7B1Ctpd7OCmMNa2pgvCItIAogicScb7n7DLAoYuih6yvQ896jZYC62S3ahAAcCXpcK02HLowoS9yar0Y-Ke9EWP8cO8zADYPFxdsrK7gkxO6HVWTun5/s1600/ideas.jpg" height="318" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. This is a rough sketch of my simple idea of why I increased my sampling to forty samples than twenty. The graphs show how outliers can ruin a graph. The orange bar graph is an ideally great graph, other than, the blue bar graph with horrific error bars.</td><td class="tr-caption" style="text-align: center;"><br /></td><td class="tr-caption" style="text-align: center;"><br /></td><td class="tr-caption" style="text-align: center;"><br /></td></tr>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I’m, also, trying to
find a way to make media with hand washing antimicrobial chemicals like triclosan
and chloroxylenol that are the active ingredient in antibacterial soaps. The
main antimicrobial chemical is chloroxylenol by <i style="mso-bidi-font-style: normal;">GOJO</i> in soap dispensers in bathrooms around campus. It used to be triclosan
ingredient soap dispensers but the school switched it out for reasons like not
foaming when dispensed, financially expensive, or other reasons. Once the media
of TSA plus antimicrobial is achieved, I will start inoculating swab samples to
test whether or not microbes are resistant to soap dispenser ingredients of
either triclosan or chloroxylenol. Figure 2 below shows the soap
dispenser around school campuses bathrooms.</span></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj5N1I1IeGOJh4_M80zD8ZZ8_035OT8-bhlIwQz-GPcB-Mpd0YOW59pQRxGM1EUQoHz6OSZwKQXxa9gy_3IxZbojLniQEp_PSp3Q__5BpRUPxVj4Yy5SHaaOsAtTFna4DhCur5phz9beZnE/s1600/soap_dispenser.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj5N1I1IeGOJh4_M80zD8ZZ8_035OT8-bhlIwQz-GPcB-Mpd0YOW59pQRxGM1EUQoHz6OSZwKQXxa9gy_3IxZbojLniQEp_PSp3Q__5BpRUPxVj4Yy5SHaaOsAtTFna4DhCur5phz9beZnE/s1600/soap_dispenser.jpg" height="400" width="307" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. This gave me idea to sample the grey button in on this soap dispenser in bathrooms. The bag inside can hold up to 1250 ml of antibacterial soap, and 1700 uses of one bag. </td></tr>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">This whole week I came
in and realized that I had to prepare media, sterile test tubes, and other research
material needed for my project.<span style="mso-spacerun: yes;"> </span>Spraying
of chemicals, like preservatives on the cadavers was performed. This would
prevent saprobe microbes from feeding on them. Helping Kim set up labs was,
also, done. Making TSA plates from the receipt of Jose and Josh was learned
this week. I would say it was very simple, and I thought it would be a more
complex lab set up. I was wrong. I was mentioning to post this picture of art
on a TSA plate, but never had time. Figure 3 is the picture
after of inoculation of two microbes that I haven’t identified.<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgUMGPMQQ6Mj5zXnFK7SVuOKMe4ik-lyXvoagTgezBCZLcEgxLOquOiTbpv_rVVxFBsewGmHBQOS_egivVZqK4ebQnQU1HEz35E8FK0U3yggP9l5xAxAJXUgbPYc9Qj9C4H0riACXvQyY4a/s1600/PICT0912.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgUMGPMQQ6Mj5zXnFK7SVuOKMe4ik-lyXvoagTgezBCZLcEgxLOquOiTbpv_rVVxFBsewGmHBQOS_egivVZqK4ebQnQU1HEz35E8FK0U3yggP9l5xAxAJXUgbPYc9Qj9C4H0riACXvQyY4a/s1600/PICT0912.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. This is made from two microbes. I would guess that the white one is S. epidermis and the orange is still unknown, and I could be wrong. </td></tr>
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Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-68197523816497371852013-03-21T21:20:00.001-07:002013-03-21T21:29:59.450-07:00Pardon My Absence<!--[if gte mso 9]><xml>
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<![endif]--><span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I felt bad for not coming in this week for my
internship, but I couldn’t find a last minute baby sitter for my children. My oldest
had a week off from school for spring break, and the youngest was sort of sick.</span><br />
<br />
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">On the other hand, I
did get a lot done today on the deadline for blogging and discussion board entries.
I, also, had time to be ahead of the curve for my microbiology lab assignment known
as the “Case Study Report of an Unknown Specimen.” Basically, I got a labeled
test tube, marked by the number seven, and received a patient information form
and reason for her reason at the hospital before my spring break. Figure 1
shows the patient form, and Figure 2 show the unknown test tube labeled “7” for further testing.</span><br />
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjcJHV5rs3XTDs_UCrTMdMone6IpMiLeNYCL930tfRcfEVM7a10hsLtcyHm5kW0NGSaOE-2jKxH81Rbb0cea9ZeVtSL9s-9AB7UySaWP7wwTujhLCiW14HUL-bY-LhExWRkYkM1YsGt6D9B/s1600/PICT0923.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjcJHV5rs3XTDs_UCrTMdMone6IpMiLeNYCL930tfRcfEVM7a10hsLtcyHm5kW0NGSaOE-2jKxH81Rbb0cea9ZeVtSL9s-9AB7UySaWP7wwTujhLCiW14HUL-bY-LhExWRkYkM1YsGt6D9B/s1600/PICT0923.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 1. The case study form was sort of funny because they added that her past medical history stated " a single mother on welfare." Why is it logical to know this information?</td></tr>
</tbody></table>
<div class="separator" style="clear: both; text-align: center;">
</div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgtMzAzbh3goZyDlX3IYpJm9Xyy_a9dGSWTxa6VORGHKSJTAd1_4389QASysqnuX0OOhyMVH_lrfaJ0ts1CzrK30Yuox3S8xPdIVk93Zo_64ryi1b8JQQ39u-iIpl2wAC4Mdw2EiwsH-Bmf/s1600/PICT0925.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgtMzAzbh3goZyDlX3IYpJm9Xyy_a9dGSWTxa6VORGHKSJTAd1_4389QASysqnuX0OOhyMVH_lrfaJ0ts1CzrK30Yuox3S8xPdIVk93Zo_64ryi1b8JQQ39u-iIpl2wAC4Mdw2EiwsH-Bmf/s1600/PICT0925.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. The test tube from left to right is the unknown microbe and the other tube is the "Mannitol Fermentation Broth Test." This test shows the by product of it's nutrients that it produced and the color indicator showed that it fermentated acid but no gas in the inner tube "Duram tube."</td></tr>
</tbody></table>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">My job was to identify
the unknown microorganism and what measure of antibiotics to kill the pathogen.
I performed techniques like inoculation of my unknown microbe to isolation
media’s like MacConkey Agar (MAC) or Mannitol Salt Agar (MSA) plates. These types
of media allow for selective and differential growth of either gram negative or
gram positive groups of bacteria to grow and thrive depending on the media. For
example, I inoculated my unknown to MAC media and nothing happened because MAC
media is for gram negative bacteria. My conclusion was that my unknown was a
gram positive bacteria, and further lab techniques like smear preparation and
gram staining gave me a view of my bacteria under the microscope for selecting
the correct Dichotomous key. Lastly, the biochemical tests on the key narrowed
the identification of the microbe</span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<br /></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">Figure 3 below shows the
final lab for this case study of identifying the right antibiotic to diminish this
foreign microbe causing this infection. This plate is called a Mueller Hinton plate with a lawn of Staphylococcus aureus.</span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<br /></div>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhdC7jTd3iME_ovCOdOM0UHG3p4XLGNa1XErcWe5it72Sf0ZtwdeDIdtstSGLMyFf5dH9irZPw_hHTm0PVTtQIZQY4s5TYSWwDJngGQRAh6yj6roT7PABKifErySdREV6uQe-vSVDaWtfRm/s1600/PICT0920.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhdC7jTd3iME_ovCOdOM0UHG3p4XLGNa1XErcWe5it72Sf0ZtwdeDIdtstSGLMyFf5dH9irZPw_hHTm0PVTtQIZQY4s5TYSWwDJngGQRAh6yj6roT7PABKifErySdREV6uQe-vSVDaWtfRm/s1600/PICT0920.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 3. Clockwise from the top left corner is a small white disk of antibiotics in the center of the quadrants, respectively: ampicillian 10 micrograms, vancomycin 30 micrograms, erythromycin 15 micrograms, and, lastly, penicillin 10 micrograms.</td></tr>
</tbody></table>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I’m currently about two
weeks ahead, and started writing my report because I began identifying the
antibiotic I’m going to use. This assignment is relevant to the STEM program
because I will have more time to spend in the internship.</span></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I had help from Matt
earlier today on how to approach the modification of my project I plan to continue
but with alterations. For instance, the previous project bar graph had unacceptable
error bars of my standard deviation. I tweaked with my data by eliminating the outliers
in a statistical view. Graph 1 below show the new and improved bar graph with
proper error bars.</span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhQbSXNiv3z-zGh8tdSBK1Rmbi3it1ElCxjLzFjK8uZOrgFla5QlNWPhgYp67_362wnNfFU3axWBe6JPpJQQqomEQ_UF5ASbiHhDTJqVUkVrlvpb0Ses0hnr-GP6oTFMw-wvHSG17udDhyphenhyphenu/s1600/Graph_Blog.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhQbSXNiv3z-zGh8tdSBK1Rmbi3it1ElCxjLzFjK8uZOrgFla5QlNWPhgYp67_362wnNfFU3axWBe6JPpJQQqomEQ_UF5ASbiHhDTJqVUkVrlvpb0Ses0hnr-GP6oTFMw-wvHSG17udDhyphenhyphenu/s1600/Graph_Blog.jpg" height="260" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Graph 1. The error bars are within the bars and I combined first and second attempt objects like the rails and elevators as one bar. I, also, excluded data like zero's and outliers from the data I collected to correct the error bars. </td></tr>
</tbody></table>
<div class="MsoNormal" style="text-indent: .5in;">
<br /></div>
<div class="MsoNormal" style="text-indent: .5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt; line-height: 115%;">I, also, still plan to
sample objects around the campus like the bathroom faucets and their door
handles, cafeteria tables, and other possible areas of contact. Instead of
sampling twenty objects of classroom desks, etc., I will, unfortunately, sample
forty objects to prevent abnormal error bars. I'm still brainstorming on how to approach the antimicrobial of microbes around the campus.</span></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-26028338918802387862013-03-07T20:44:00.000-08:002013-03-07T20:47:26.325-08:00No Pictures Please<!--[if gte mso 9]><xml>
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<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjUNF32USq-7Ym7hCHa-A8WoBXcXomjw9WhRZEW78QUHPpyPvbDR1S20uCwUijYqxEy7_RgV6dQzeaD_vZ3onGXec9SAIbvKSEdpxfnrrPbchnwxftSPyq643Kki1R3_ZKBOBF0xi5HQn22/s1600/5jeOx4ZfhDnx4dTLMcaTfsGa6XF4nYGhaTqZMd1Gd3MkfZQxSkMOQSHLNBwaMY7gX.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjUNF32USq-7Ym7hCHa-A8WoBXcXomjw9WhRZEW78QUHPpyPvbDR1S20uCwUijYqxEy7_RgV6dQzeaD_vZ3onGXec9SAIbvKSEdpxfnrrPbchnwxftSPyq643Kki1R3_ZKBOBF0xi5HQn22/s1600/5jeOx4ZfhDnx4dTLMcaTfsGa6XF4nYGhaTqZMd1Gd3MkfZQxSkMOQSHLNBwaMY7gX.jpg" height="266" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure1. I look so tired in this picture, and it was posted on the website. Here's a link to view other peoples picture on the website. I think I saw Jose's Pic </td><td class="tr-caption" style="text-align: center;"><br /></td></tr>
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<br />
<div class="MsoNormal" style="line-height: normal; margin-bottom: .0001pt; margin-bottom: 0in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt;"><span style="mso-tab-count: 1;"> <a href="http://mati.eas.asu.edu/mgeonwp/?p=317" target="_blank">Click Here for Other Pictures</a></span></span></div>
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<br /></div>
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<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt;"><span style="mso-tab-count: 1;"> </span>I finished my poster last Friday on
the first day of March, and started it the day before on Thursday. The second
day working on my poster was for modifications and changing of the format
style. Although I didn’t get the nice glossy thick paper like the other
students poster, I had my poster printed out in Student Union on regular paper.
The reason for my low grade paper texture was my failure to turn my rough draft
on time for peer review. On the other hand, it was free of charge thanks to an
office coordinator of the <i style="mso-bidi-font-style: normal;">Student Life
and Leadership</i>. </span></div>
<div class="MsoNormal" style="line-height: normal; margin-bottom: .0001pt; margin-bottom: 0in; text-indent: .5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt;">Prior
to attending the conference for my poster presentation, I had been nervous the
whole month the application had been submitted online. When I got there, it was
simple. I thought, for example, that I would present on stage with my poster
followed by a speech. I admit that I learned a lot from this experience before
and after the ASU student research conference of <i style="mso-bidi-font-style: normal;">More Graduate Education at Mountain States Alliance</i> (MGE@MSA). The
first lesson learned is always be prepared for the unexpected and identify the
highest priority. I should have been more organized in prioritizing my
assignments on my agenda. Second, never use obstacles in life as an excuse for
failure. The realization is that I do have a family, school, and internship,
but my determination can exceed further and I can work harder. Thirdly, I should
have not judge the event without having prior conference experience. This
student research conference was, for instance, my first experience. Overall,
the atmosphere of determined, intelligent, and science related college students
of undergraduates and doctorate’s was interesting. I glanced at a few posters,
and had a judge give advice on some helpful tips on my continued research.
Moreover, they had pretty decent lunches during the lunch hour and breakfast. I
really enjoyed the humorous ASU instructor remarks about “Why it’s important to
pursue higher education?” I liked his story about how he grew up in a bad part
of New York, and now he has a family to support that he couldn’t have dreamed about
due, in part, to his father’s pushing mentor ways. The most hardest thing I found hard at the conference was the post registration of obtaining the Certificate of Participation.</span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgkKSPDPz4GR1WvVJgIkmTQppJZPVpkvg7QpcRtXbIueF_V_-GXPdTPbSSMvHdCEFyWLCw6-c_6v4j50ebUPwj7zLa0FUKeCrEnUWGc09_a3xykhIjj4Azoc2U252-G1352yPmjnuqLtK7E/s1600/2012-02-14-682.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgkKSPDPz4GR1WvVJgIkmTQppJZPVpkvg7QpcRtXbIueF_V_-GXPdTPbSSMvHdCEFyWLCw6-c_6v4j50ebUPwj7zLa0FUKeCrEnUWGc09_a3xykhIjj4Azoc2U252-G1352yPmjnuqLtK7E/s1600/2012-02-14-682.jpg" height="265" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Figure 2. This picture shows similar details of the long lines and post registration of last years conference.</td></tr>
</tbody></table>
<div class="MsoNormal" style="line-height: normal; margin-bottom: .0001pt; margin-bottom: 0in; text-indent: .5in;">
<span style="font-family: "Times New Roman","serif"; font-size: 12.0pt;">I
have a question for my fellow colleagues of the STEM Scholar program. Have you
taken that psychological test online that one of the speakers had on the Power Point
of your true intentions in academic school work? Basically, if your smart or do
you act like it?</span></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0tag:blogger.com,1999:blog-3022094937571737801.post-33298667103051302292013-02-28T23:45:00.003-08:002013-02-28T23:45:18.813-08:00Different Day, New Challenges<div class="separator" style="clear: both; text-align: center;">
<iframe allowfullscreen='allowfullscreen' webkitallowfullscreen='webkitallowfullscreen' mozallowfullscreen='mozallowfullscreen' width='320' height='266' src='https://www.youtube.com/embed/eovTbQgan5M?feature=player_embedded' frameborder='0'></iframe></div>
<br />
<div class="MsoNormal" style="margin: 0in 0in 8pt;">
<span style="font-family: Calibri;"><span style="mso-tab-count: 1;"> </span>I
finally got the paper work done for the Honor’s Program, and I don’t feel any
different. Although the semester is still a challenge, I feel the A’s in MAT
182, BIO 205 and lab, REL 245, SPH 243, and CHM 235 lab is appreciation to keep
moving forward. I feel the ASU conference poster is almost done even if I did
barely started on it today. I learned a lot this week. For example, the
microbiology lab was very interesting. I, basically, took DNA from E. coli,
poopy bacteria, and integrated it with a plasmid. The realization was that the
chemical calcium chloride used to open the pores of the bacteria wall had
significance to my previous class of general chemistry 151 and 152. The
characteristic of calcium chloride was that at room temperature it was a liquid
like water, and it became crystalized when stored in 4 degrees Celsius. The
bond between the chemical and the cell wall fused, so the solution was put into
ice bath. It left holes large enough for the plasmid to enter the cells
cytoplasm and either integrate with its DNA chromosome or remain a plasmid.
This plasmid had instructions for antibiotic resistance against ampicillin.
This is known as Transformation for bacteria to recombine their genes as one
way.<o:p></o:p></span></div>
<br />
<div class="MsoNormal" style="margin: 0in 0in 8pt;">
<span style="font-family: Calibri;"><span style="mso-tab-count: 1;"> </span>I,
also, taught myself to change an alternator on my vehicle. I am fitting
everything into my schedule. This past month I have been fixing a constant SUV
with problems after problems; thus, learning new things about cars and trying
to spend time with my son and eager to learn daughter is a busy semester this
year. I feel like the time I spend with my kids and companion is shorting every
week. I countdown the weeks every month, so there is about half a semester
left. I haven’t put much time into the internship this week because of the
hectic schedule. The poster I, somewhat, finished seems to boring. There is too
much words and not enough pictures. The average person spends 3 minutes or less
reading the first part of a book before they read anymore. I looked at my
poster and realized it something I wouldn’t read because it’s too rambling. I,
also, haven’t been putting too much effort into O-CHEM. I would say this class
is going to be either a “B” grade or “C.” I don’t grasp what the instructor is
saying, maybe because there is a language barrier. I’m dealt the cards and I
must play my hand. I guess? I read a book for a class called <i style="mso-bidi-font-style: normal;">Bless Me, Ultima</i>. A Chicano literature
about a boy growing up and questioning life. The movie is, also, in theaters.<o:p></o:p></span></div>
Darren Johnsonhttp://www.blogger.com/profile/04832443936076067693noreply@blogger.com0